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Unless necessary ATCC recommends. For cultures that require storage in liquid nitrogen it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen.
Unless necessary ATCC recommends.

. Upon thawing the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. For cultures that require storage in liquid nitrogen it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris.
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